Ascorbic acid uptake to isolated nerve terminals and secretory granules from ox neurohypophyses

Publisher Website
Google Scholar
Abstract
Isolated nerve terminals (neurosecretosomes) from cow neurohypophyses accumulated radioactivity when they were incubated with L[14C]-ascorbic acid in an ionic medium dominated by NaCl. Uptake of radioactivity was saturable with ascorbic acid concentration. Replacement of Na+ with Li+ in the incubation medium or presence of ouabain inhibited the accumulation. Isolated, purified cow neurosecretory granules contained 14 +/- 2 nmol ascorbate (n = 10) per mg of protein. When such granules were incubated with L[14C]-ascorbic acid in a KCl dominated medium, they took up radioactivity slowly. The accumulation was not saturable with ascorbic acid concentration and was not influenced by the presence of Mg2+ATP.