Control of Dimorphism in the Female Honeybee. 3. The Balance of Nutrients

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Abstract
Pure nutrients or fractions of royal jelly were added to whole royal jelly, which was then used to rear larvae to the adult stage in the laboratory. Larvae grew and differentiated almost (if not quite) normally when large quantities of glucose, fructose, sucrose, several water-soluble organic acids, a mixture of essential amino acids, or a vitamin mixture, were added to the diet. Larvae were tolerant of fairly high concentrations of royal jelly protein and smaller concentrations of royal jelly lipids. They grew and differentiated when most constituents of royal jelly were diluted to 66% of their normal concentration. Some of the non-essential amino acids, some organic acids, and com oil were deleterious at low concentrations. Larvae were fairly sensitive to some inorganic salts, and care must be exercised to ensure that excess salt is not introduced into laboratory diets.