Guanidinium-CsCl Density Gradients for Isopycnic Analysis of Nucleic Acids
- 7 November 1975
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 190 (4214) , 584-586
- https://doi.org/10.1126/science.1188358
Abstract
The addition of guanidinium chloride to CsCl gradients lowers the apparent density of RNA, DNA, and hybrid polymers in such a way that all three can be banded, fractionated, and analyzed in one gradient with essentially no damage to their chemical integrity or to their biological activity.Keywords
This publication has 12 references indexed in Scilit:
- Sensitivity of Escherichia coli to Viral Nucleic AcidThe Journal of Biochemistry, 1972
- Buoyant density gradient centrifugation of RNA and DNA in alkali iodide solutionsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- The buoyant behavior of RNA and DNA in cesium sulfate solutions containing dimethylsulfoxideBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- [103a] The use of guanidinium chloride in the isolation of nucleic acidsPublished by Elsevier ,1968
- Equilibrium centrifugation of DNA, RNA and poliovirus in density gradients of cesium oxalate and cesium acetateBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- Fractionation of viral-specific ribonucleic acid by cesium sulfate equilibrium density-gradient centrifugationJournal of Molecular Biology, 1966
- The evaluation of standard sedimentation coefficients of sodium RNA and sodium DNA from sedimentation velocity data in concentrated NaCl and CsCl solutionsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- The replication of bacteriophage MS2Journal of Molecular Biology, 1965
- The molecular conservation of ribonucleic acid during bacterial growthJournal of Molecular Biology, 1960
- The Thermal Denaturation of Desoxyribose Nucleic AcidJournal of the American Chemical Society, 1957