THE ISOLATION OF THE ANTIBODY MOIETIES OF IMMUNE-COMPLEXES FROM SERUM BY THE PEPSIN DIGESTION OF CONGLUTININ-ANTI-CONGLUTININ COMPLEXES
- 1 January 1981
- journal article
- research article
- Vol. 46 (2) , 250-258
Abstract
A technique is described which allows the antibodies of circulating immune complexes to be isolated as their F(ab'')2 fragments. The method is based on the precipitation of the complexes by the sequential addition of conglutinin and anti-conglutinin and the subsequent digestion of these precipitates by pepsin. Using this technique it was possible to show antibodies to Epstein-Barr(EB) virus antigens in the immune complexes of patients with Burkitt''s lymphoma and to microbial antigens in 2 patients with nephritis. By substituting DNase for pepsin it was also possible to show antibodies to DNA-containing nuclear antigens in the serum of patients with systemic lupus erythematosus.This publication has 5 references indexed in Scilit:
- Conglutinin binding polyethylene glycol precipitation assay for immune complexes.1979
- PURIFICATION OF SOLUBLE IMMUNE-COMPLEXES FROM SERUM USING POLYMETHYLMETACRYLATE BEADS COATED WITH CONGLUTININ OR CLQ - APPLICATION TO THE ANALYSIS OF THE COMPONENTS OF INVITRO FORMED IMMUNE-COMPLEXES AND OF IMMUNE-COMPLEXES OCCURRING INVIVO DURING LEISHMANIASIS1979
- Activation of the alternative complement pathway by human B cell lymphoma lines is associated with Epstein‐Barr virus transformation of the cellsEuropean Journal of Immunology, 1978
- The purification of specific antibody as F(ab′)2 by the pepsin digestion of antigen-antibody precipitates, and its application to immunoglobulin and complement antigensImmunochemistry, 1971
- A COMPARISON OF SOME PROPERTIES OF BOVINE CONGLUTININ WITH THOSE OF RABBIT IMMUNO-CONGLUTININ1962