Na+-coupled glycine transport in reticulocyte vesicles of distinct sidedness: Stoichiometry and symmetry

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Abstract
Sodium-coupled glycine transport has been studied using membrane vesicles of distinct sidedness, either inside-out or right side-out, prepared from sheep reticulocytes. The activity is chloride dependent and characterized by high and low apparent affinities for glycine (K′ m ≅0.5mm and >10mm) for both types of vesicles as well as intact cells. Transport is symmetrical with respect to similar apparent affinity constants for glycine, for both the high- and low-affinity systems, and for sodium. Direct measurements of the sodium/glycine coupling indicate a ratio of 2∶1, consistent with kinetic data fitted to a Hill-type equation describing glycine flux as a function of sodium concentration.

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