Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging
- 1 February 2003
- journal article
- Published by Optica Publishing Group in Journal of the Optical Society of America A
- Vol. 20 (2) , 368-379
- https://doi.org/10.1364/josaa.20.000368
Abstract
We deduce the signal-to-noise ratio for fluorescence lifetime imaging when using frequency-domain methods. We assume mono-exponential decay and quantum-noise-limited performance. The results are compared with Monte Carlo simulations with good agreement. We also compare our results with previous investigations of time-domain methods for fluorescence lifetime imaging. For a given number of detected photons, we find that frequency-domain and time-domain methods are equally good. The correct choice of detection technique and its parameters is important for obtaining good results.Keywords
This publication has 23 references indexed in Scilit:
- Fluorescence lifetime-based sensing of pH, Ca2+, K+ and glucosePublished by Elsevier ,2001
- Multiple Time-Gate Module for Fluorescence Lifetime ImagingApplied Spectroscopy, 2001
- Time‐gated fluorescence lifetime imaging and microvolume spectroscopy using two‐photon excitationJournal of Microscopy, 1998
- Double‐pulse fluorescence lifetime measurementsJournal of Microscopy, 1997
- Fluorescence lifetime measurements in confocal microscopy of neurons labeled with multiple fluorophoresNature Biotechnology, 1997
- Confocal fluorescence microscopy using spectral and lifetime information to simultaneously record four fluorophores with high channel separationJournal of Microscopy, 1997
- Micro-Volume Time-Resolved Fluorescence Spectroscopy Using a Confocal Synchrotron Radiation MicroscopeApplied Spectroscopy, 1995
- Fluorescence lifetime imaging using a confocal laser scanning microscopeScanning, 1992
- Ein Fluorometer. Apparat zur Messung von FluoreszenzabklingungszeitenThe European Physical Journal A, 1927