Syntheses of Labeled Vitamers of Folic Acid to Be Used as Internal Standards in Stable Isotope Dilution Assays
- 23 July 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 50 (17) , 4760-4768
- https://doi.org/10.1021/jf025571k
Abstract
[2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays. Keywords: Electrospray mass spectrometry; folates; labeled folic acid; stable isotope dilution assayKeywords
This publication has 19 references indexed in Scilit:
- Determination of 5-Methyltetrahydrofolic Acid in Human Serum by Stable-Isotope Dilution High-Performance Liquid Chromatography–Mass SpectrometryAnalytical Biochemistry, 2001
- A Quantitative Stable-Isotope LC−MS Method for the Determination of Folic Acid in Fortified FoodsJournal of Agricultural and Food Chemistry, 2001
- Standardisation of HPLC techniques for the determination of naturally-occurring folates in foodFood Chemistry, 1999
- Determination of Folate in Cereal-Grain Food Products Using Trienzyme Extraction and Combined Affinity and Reversed-Phase Liquid ChromatographyJournal of Agricultural and Food Chemistry, 1997
- Comparison of a HPLC and radioprotein-binding assay for the determination of folates in milk and blood samplesFood Chemistry, 1995
- Preparation of [2′,3′,5′,6′‐2H4]pteroylglutamic acidJournal of Labelled Compounds and Radiopharmaceuticals, 1995
- Chemical synthesis of deuterated folate monoglutamate and in vivo assessment of urinary excretion of deuterated folates in manAnalytical Biochemistry, 1988
- A simple procedure for the synthesis of high specific activity tritiated (6S)-5-formyltetrahydrofolateAnalytical Biochemistry, 1982
- [75] Synthesis and biological evaluation of 2-amino-4-hydroxy-6-hydroxymethylpteridine pyrophosphatePublished by Elsevier ,1980
- Preparation and crystal structure of 6-acetyl-1-iodocodeineThe Journal of Organic Chemistry, 1978