Amino Acid Requirements of Acetobacter suboxydans

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Abstract
The organism multiplies to a limited extent in a medium consisting of glycerol, salts, essential growth factors, and a combination of valine, isoleucine, alanine, and histidine as-N source. Growth is considerably improved by the addition of either cystine or methionine. The further addition of proline increases growth to the level obtainable with a mixture of 20 amino acids or hydrolyzed casein. However, development is not so rapid and somewhat less extensive than with yeast extract. At suboptimum concs. of the 6 required amino acids, (NH4)2SO4 stimulated growth. Resting cell suspensions of A. suboxydans deaminate most amino acids under aerobic conditions. Both optical isomers of alanine, serine, and aspartic acid are attacked. Under anaerobic conditions only serine is deaminated to any appreciable extent.

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