Affinity Chromatography of Phenylalanine: tRNA Ligase
- 1 March 1973
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 51 (3) , 231-234
- https://doi.org/10.1139/o73-029
Abstract
L-Phenylalanine: tRNA ligase from E. coli was successfully isolated by affinity chromatography on a column of Sepharose-phenylalanine. The phenylalanine was separated from the Sepharose backbone by two molecules of ε-aminocaproic acid to ensure that there was a minimum of steric interference between the enzyme and the solid matrix.Keywords
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