Crossed immuno‐electrophoretic analysis of Yersinia enterocolitica serotype 0:3 antigens
- 1 January 1988
- Vol. 96 (1-6) , 306-314
- https://doi.org/10.1111/j.1699-0463.1988.tb05307.x
Abstract
By means of quantitative immuno‐electrophoretic methods, 81 different antigens were demonstrated in sonicated preparations of Yersinia enterocolitica, serogroup 0:3, using corresponding rabbit antiserum. Only chromosomally‐encoded antigens were demonstrated in this system. Thirteen antigens were located on the bacterial surface, as demonstrated after absorption of rabbit antiserum with whole, killed bacteria. Five antigens resisted boiling. Antigen No. 71 was identified as an important part of the lipopolysaccharide, and antigen No. 6 as the “common antigen” of Pseudomonas aeruginosa. Binding activities to concanavalin A (indicating the presence of carbohydrates), to Limulus amoebocyte lysate and to polymyxin B (indicating the presence of possible endotoxin activity) were demonstrated for five, nine, and seven antigens, respectively. These observations, together with the presence of corresponding antibodies in pre‐immune rabbit sera, as well as cross‐reactivity with other Gram‐negative bacteria, indicate the biological importance of several of these antigens.Keywords
This publication has 26 references indexed in Scilit:
- Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharidesPublished by Elsevier ,2003
- Cross‐reactions between Yersinia enterocolitica serogroup 0:3 and other serogroups of the same species, as well as thirty‐four other bacterial speciesAPMIS, 1988
- Monoclonal antibodies directed against plasmid-encoded released proteins of enteropathogenicYersiniaFEMS Microbiology Letters, 1986
- Outer Membrane Proteins and Plasmids in Different Yersinia Enterocolitica Serogroups Isolated from Man and AnimalsActa Pathologica Microbiologica Scandinavica Series B: Microbiology, 1985
- Pathogenesis of Yersinia‐Triggered Reactive Arthritis: Immunological, Microbiological and Clinical AspectsImmunological Reviews, 1985
- QUANTITATIVE IMMUNOELECTROPHORETIC ANALYSIS OF SALMONELLA TYPHI ANTIGENS AND OF CORRESPONDING ANTIBODIES IN HUMAN SERAActa Pathologica Microbiologica Scandinavica Section B Microbiology, 1980
- IDENTIFICATION AND QUANTITATION OF PRECIPITINS AGAINST PSEUDOMONAS AERUGINOSA IN PATIENTS WITH CYSTIC FIBROSIS BY MEANS OF CROSSED IMMUNOELECTROPHORESIS WITH INTERMEDIATE GELActa Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology, 1973
- Lectins: Cell-Agglutinating and Sugar-Specific ProteinsScience, 1972
- Méthode permettant l'étude conjuguée des propriétés électrophorétiques et immunochimiques d'un mélange de protéines. Application au sérum sanguinBiochimica et Biophysica Acta, 1953