Direct, fixed-time kinetic assays for beta-hydroxybutyrate and acetoacetate with a centrifugal analyzer or a computer-backed spectrophotometer.
Open Access
- 1 November 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 26 (12) , 1713-1717
- https://doi.org/10.1093/clinchem/26.12.1713
Abstract
In the course of studying the control of blood glucose in juvenile onset diabetics, we developed convenient methods for determining beta-hydroxybutyrate and acetoacetate. Here we describe fixed-time, enzymic, reaction-rate procedures for directly measuring these organic acids with a centrifugal analyzer (Rotochem IIA/36) or a computer-backed spectrophotometer (Gilford 102 system). In either case, the method requires only 20 micro L of plasma; is rapid, accurate, and precise; and analytical recovery is quantitative. Data are presented comparing results obtained with both instruments. Metabolic acidosis can be rapidly assessed and monitored with these methods, as illustrated by an example.Keywords
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