Rapid, single-step purification of restriction endonucleases on Cibacron Blue F3GA-agarose
- 1 October 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (20) , 4136-4139
- https://doi.org/10.1021/bi00613a005
Abstract
After sonication and high-speed centrifugation, crude extracts of Bacillus amyloliquefaciens, Providencia alcalifaciens, Xanthomonas holicola and B. globigii were adsorbed on the dye Cibacron blue F3GA covalently cross-linked to agarose. The restriction endonucleases BamHI, PalI, XhoI and Bg/I together with BglII were isolated by elution of the dye column with linear gradients to 0.5 M NaCl. The enzymes so purified were free of contaminating nucleic acids and other nucleases and were sufficiently concentrated for direct, specific DNA hydrolysis.This publication has 2 references indexed in Scilit:
- Applications of blue dextran and cibacron blue F3GA in purification and structural studies of nucleotide-requiring enzymesBiochemical and Biophysical Research Communications, 1976
- A specific endonuclease from Arthrobacter luteusJournal of Molecular Biology, 1976