Characterization of the Carbohydrate Moiety in a Purified Allergen Preparation from the Mite Dermatophagoides Farinae and Its Importance for Allergenic Activity as Tested by the RAST-Inhibition Method

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Abstract
The monosaccharide composition of a purified mite allergen preparation was mainly found to be: mannose, galactose, glucose, N-acetylglucosamine and N-acetylgalactosamine quantitated to 15% of dry material by means of methanolysis and GLC of trimethylsilylated methyl glycosides. Protein content was found to be 65%, determined by the Lowry method. Methylation analysis and periodate oxidation indicated the main polysaccharide material to be built of 1,6-linked hexosamine, with side chains having mainly galactose end groups. Attempts to obtain separate carbohydrate fractions by ion exchange chromatography of the extract on CM-Sephadex and DEAE-Sephadex columns were not successful. The RAST-inhibition test showed a lower IgE binding capacity for periodate-treated mite preparation, where sugars containing vicinal diols have been oxidized, compared to an untreated mite preparation.

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