Field Inversion Gel Electrophoretic Separation of Cryptosporidium spp. Chromosome-Sized DNA

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Abstract
Chromosomal DNA from 5 isolates of Cryptosporidium parvum and 1 of Cryptosporidium baileyi were compared by field-inversion gel electrophoresis (FIGE). FIGE analyses of parasite DNA prepared from purified sporozoites versus intact oocysts showed no observable differences. Chromosmal DNA migration patterns of the 5 C. parvum isolates were indistinguishable, whereas similar but distinct differences were evident between C. baileyi and the isolates of C. parvum. Oocyst-reactive monoclonal antibodies differentiated oocysts of C. parvum from those of C. baileyi but were unable to distinguish oocytes of 1 isolate of C. parvum from another.