Beta 1 integrin-mediated interaction with extracellular matrix proteins regulates cytokine gene expression in synovial fluid cells of rheumatoid arthritis patients.
Open Access
- 1 March 1993
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 177 (3) , 863-868
- https://doi.org/10.1084/jem.177.3.863
Abstract
Inflammatory cytokines have been implicated in the pathogenesis of rheumatoid arthritis (RA), whereas the mechanisms for constitutive production of inflammatory cytokines in affected joints are largely unknown. Recently, integrin-mediated interaction with extracellular matrix (ECM) proteins has been demonstrated to play a role in regulating cytokine production in T cells and monocytes. In this study, we investigated the contribution of the beta 1 integrin-mediated interaction with ECM proteins to the persistent cytokine gene expression in RA synovial fluid mononuclear cells (SFMNC). We examined mRNA expression of 14 cytokines in the SFMNC of three RA patients, which were either fresh or cultured overnight in serum-free medium on ECM-coated plates, by polymerase chain reaction with a panel of oligonucleotide primers specific for each cytokine. The persistent expression of various cytokine mRNA found in fresh SFMNC was maintained after overnight culture in serum-free medium on ECM proteins, especially on laminin (LM), but not on serum albumin. This effect of LM was inhibited by an anti-integrin beta 1 chain (CD29) mAb, as well as by an anti-CD3 mAb, indicating an important role of the beta 1 integrin-mediated interaction with ECM proteins in regulating persistent cytokine gene expression in RA SFMNC, and a key role of T cells in regulating inflammatory monokine production.Keywords
This publication has 33 references indexed in Scilit:
- Induction of human hsp60 expression in monocytic cell linesInternational Immunology, 1992
- Accessory Cell Signals Involved in T‐Cell ActivationImmunological Reviews, 1990
- Detection of interleukin 8 biological activity in synovial fluids from patients with rheumatoid arthritis and production of interleukin 8 mRNA by isolated synovial cellsEuropean Journal of Immunology, 1990
- Rheumatoid ArthritisNew England Journal of Medicine, 1990
- VLA Proteins in the Integrin Family: Structures, Functions, and Their Role on LeukocytesAnnual Review of Immunology, 1990
- Lymphocyte Extracellular Matrix Interactions.Scandinavian Journal of Immunology, 1989
- Clonal Expansion Versus Functional Clonal Inactivation: A Costimulatory Signalling Pathway Determines the Outcome of T Cell Antigen Receptor OccupancyAnnual Review of Immunology, 1989
- Interleukin 2 responsive T cell clones from rheumatoid and normal subjects: Proliferative responses to connective tissue elementsClinical Immunology and Immunopathology, 1989
- The american rheumatism association 1987 revised criteria for the classification of rheumatoid arthritisArthritis & Rheumatism, 1988
- Very late activation antigens on rheumatoid synovial fluid T lymphocytes. Association with stages of T cell activation.Journal of Clinical Investigation, 1986