Characterization of Brucella ovis lipopolysaccharide and its use for diagnosis of ram epididymitis by enzyme-linked immunosorbent assay

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Abstract
Rough lipopolysaccharide, extracted by a mixture of phenol, chloroform and petroleum ether from freeze-dried B. ovis cells with a yield of 0.71%, contained relatively small amounts of protein and nucleic acid contaminants as compared with lipopolysaccharides from other Brucellae. The crude lipopolysaccharide was suitable as a diagnostic antigen in an enzyme-linked immunosorbent assay [ELISA] for the sensitive and specific detection of ram epididymitis caused by B. ovis infection. In comparative serological tests, the ELISA with B. ovis lipopolysaccharide gave better identification of infections and fewer false-negative results than the ELISA with sonicated antigen or the complement fixation test.