Next-generation sequencing transforms today's biology
Top Cited Papers
- 19 December 2007
- journal article
- review article
- Published by Springer Nature in Nature Methods
- Vol. 5 (1) , 16-18
- https://doi.org/10.1038/nmeth1156
Abstract
A new generation of non-Sanger-based sequencing technologies has delivered on its promise of sequencing DNA at unprecedented speed, thereby enabling impressive scientific achievements and novel biological applications. However, before stepping into the limelight, next-generation sequencing had to overcome the inertia of a field that relied on Sanger-sequencing for 30 years.Keywords
This publication has 19 references indexed in Scilit:
- Global gene expression analysis of the shoot apical meristem of maize (Zea mays L.)The Plant Journal, 2007
- High-throughput oncogene mutation profiling in human cancerNature Genetics, 2007
- Sequencing and Analysis of Neanderthal Genomic DNAScience, 2006
- The Marine Viromes of Four Oceanic RegionsPLoS Biology, 2006
- Microbial diversity in the deep sea and the underexplored “rare biosphere”Proceedings of the National Academy of Sciences, 2006
- A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomesProceedings of the National Academy of Sciences, 2006
- Comprehensive mutation identification in an evolved bacterial cooperator and its cheating ancestorProceedings of the National Academy of Sciences, 2006
- Genome sequencing in microfabricated high-density picolitre reactorsNature, 2005
- A rapid method for determining sequences in DNA by primed synthesis with DNA polymerasePublished by Elsevier ,2004
- Nucleotide sequence of bacteriophage φX174 DNANature, 1977