Isolation and Composition of the Extracellular Slime Made by Coagulase-Negative Staphylococci in a Chemically Defined Medium
- 1 March 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 163 (3) , 534-541
- https://doi.org/10.1093/infdis/163.3.534
Abstract
Slime isolated after growth of four strains of coagulase-negative staphylococcion chemically definedmedium plus agar wasrich in galactose. However, whensterile agar plates wereextracted with saline, high-molecular-weight material with similar properties was obtained that also was galactose-rich. Most of the dry weight attributed to slime, and probably all the galactose, originated from agar. Slime isolated by gel and ion-exchange chromatography from liquid culture in the same medium contained glycerol phosphate, glucose(no galactose), glucosamine, alanine, uronate, an unidentified component, and protein. Separation of protein from carbohydrate was achievedby affinity chromatography. [14C]glucose in the medium labeled the carbohydrate polymer; [14C]amino acids chiefly labeled extracellular proteins. Slime from bacteria grown on medium solidified with silica gel or on dialysis membrane abovean agar surface showed essentially the same composition and behavior after purification as the material isolated from liquid culture.Keywords
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