ACTIVATED HUMAN MONONUCLEAR PHAGOCYTES RELEASE A SUBSTANCE(S) THAT INDUCES REPLICATION OF QUIESCENT HUMAN-FIBROBLASTS

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 31  (4) , 281-293
Abstract
Human mononuclear phagocytes (HMP) treated in vitro with N-acetyl-muramyl-L-alanyl-D-isoglutamine (MDP) released a soluble substances(s) that induced in vitro replication of subconfluent, quiescent, serum-free human fibroblasts. The response was enhanced if nonmitogenic plasma-derived serum (PDS) was added to the HMP-conditioned media after harvest. The capacity of serum-free, quiescent fibroblasts to respond to the HMP-released substance(s) diminished with time in culture. The diminished proliferative response was reversed by adding PDS to the MDP-HMP-conditioned media. Similar results were obtained in parallel experiments using platelet-derived growth factor (PDGF). The HMP-associated growth-promoting substances may, like PDGF, act coordinately with substances in PDS to stimulate the proliferation of cells in vitro.