Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.
Open Access
- 1 July 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (7) , 1144-1146
- https://doi.org/10.1093/clinchem/24.7.1144
Abstract
We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.This publication has 2 references indexed in Scilit:
- Phosphatidylinositol and phosphatidylglycerol in amniotic fluid: Indices of lung maturityAmerican Journal of Obstetrics and Gynecology, 1976
- Phosphatidylglycerol in lung surfactant. III. Possible modifier of surfactant functionJournal of Lipid Research, 1976