A Comparison of Glycerol and Aqueous Methanol Equilibration for Increased Resolution during Two-Dimensional Electrophoresis

1 June 1986

journal article
research article
Published by Taylor & Francis in Separation Science and Technology

Vol. 21 (4) , 433-437
https://doi.org/10.1080/01496398608057173

Abstract

The O'Farrell method of high resolution two-dimensional electrophoresis (1) is widely used for analysis of complex protein mixtures (2, 3). Among the described modifications to this technique are changes in the sodium dodecyl sulfate (SDS) equilibration step associated with the transfer of first dimension isoelectric focusing (IEF) gels to second dimension polyacrylamide electrophoresis gels (4–7). Wright (8) has recently recommended SDS equilibration in aqueous 55% methanol for improved resolution. Since we have encountered problems applying this approach to our particular two-dimensional gel system, a simpler, rapid, and more convenient method based on IEF gel shrinkage in 100% glycerol is now recommended.

Keywords

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