DESTRUCTION OF EXPERIMENTAL MALIGNANT-MELANOMA BY MEDIATORS OF CELLULAR IMMUNITY

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (2) , 572-577
Abstract
Listeria monocytogenes (LM) in admixture with B-16 melanoma suppresses local development in syngeneic C57BL/6 mice. In vitro, LM-immune peritoneal and splenic cells are cytotoxic to B-16. Induction of cell-mediated immunity to LM antigens are required for the killing effect, since effector cells from LM-immune athymic nude mice are unable to kill tumor cells in vitro. Elimination of macrophages by a specific antiserum plus complement also abrogates the cytotoxic effect of peritoneal cells. Peritoneal or splenic adherent or nonadherent cells are not cytotoxic; combination of these two cell populations in the presence of the specific antigen can kill the B-16 target cells. A factor, probably lymphotoxin, released by the intact effector cells in the culture fluid mediates tumor cell destruction in vitro. Production of this factor requires cooperation of macrophages with specifically sensitized thymus-derived cells.

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