p53-Dependent Association between Cyclin G and the B′ Subunit of Protein Phosphatase 2A
Open Access
- 1 November 1996
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 16 (11) , 6593-6602
- https://doi.org/10.1128/mcb.16.11.6593
Abstract
We and others previously showed that cyclin G is a transcriptional target of the p53 tumor suppressor protein. However, cellular proteins which might form a complex with cyclin G have not yet been identified. To gain insight into the biological role of cyclin G, we used the yeast two-hybrid screen and isolated two mouse cDNAs encoding cyclin G-interacting proteins. Interestingly, both positive cDNAs encoded B9 regulatory subunits of protein phosphatase 2A (PP2A). One clone encodes B9alpha, while the other clone codes for a new member of the B9 family, B9beta. B9beta is 70% identical to other members of the B9 family. B9alpha associated both in vitro and in vivo with cyclin G but not with the other mammalian cyclins. Furthermore, cyclin G formed a complex with B9alpha only after induction of p53 in p53 temperature-sensitive cell lines. These results indicate that cyclin G forms a specific complex with the B9 subunit of PP2A and that complex formation is regulated by p53. Potential roles for the cyclin G-B9 complex in p53-mediated pathways are discussed.Keywords
This publication has 50 references indexed in Scilit:
- Cyclin G1 and Cyclin G2 Comprise a New Family of Cyclins with Contrasting Tissue-specific and Cell Cycle-regulated ExpressionJournal of Biological Chemistry, 1996
- High Complexity in the Expression of the B′ Subunit of Protein Phosphatase 2A0Journal of Biological Chemistry, 1996
- Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumoursNature, 1996
- Identification of a New Family of Protein Phosphatase 2A Regulatory SubunitsJournal of Biological Chemistry, 1995
- Induction of the growth inhibitor IGF-binding protein 3 by p53Nature, 1995
- Nuclear Accumulation of p53 in Normal Human Fibroblasts Is Induced by Various Cellular Stresses which Evoke the Heat Shock Response, Independently of the Cell CycleJapanese Journal of Cancer Research, 1995
- p21 is a universal inhibitor of cyclin kinasesNature, 1993
- WAF1, a potential mediator of p53 tumor suppressionCell, 1993
- Protein serine/threonine phosphatases and cell transformationBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1993
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988