DC-Chol liposomemediated gene transfer in rat spinal cord
- 1 July 1997
- journal article
- molecular neuroscience
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 8 (9) , 2355-2358
- https://doi.org/10.1097/00001756-199707070-00050
Abstract
We examined the potential of non-viral vector-mediated gene transfection in the rat spinal cord. Reporter gene (β-gal) or brain-derived neurotrophic factor (BDNF) cDNA containing a pCMV promoter complexed with DC-Chol liposomes was injected into the intact rat spinal cord gray matter. RT-PCR confirmed the increased expression of BDNF mRNA in the injection areas. X-gal staining demonstrated the localized expression of β-gal reporter genes. No overt tissue damage caused by DC-Chol liposome/DNA complex injections was detected. These results suggest that cationic liposome-mediated delivery can be a practical method for gene transfer in spinal cord.Keywords
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