Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides
- 1 January 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (13) , 2460-2465
- https://doi.org/10.1093/nar/22.13.2460
Abstract
UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.Keywords
This publication has 15 references indexed in Scilit:
- Basic matrixes for the matrix-assisted laser desorption/ionization mass spectrometry of proteins and oligonucleotidesAnalytical Chemistry, 1993
- Antisense DNA oligonucleotides II: The use of matrix‐assisted laser desorption/ionization mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotidesRapid Communications in Mass Spectrometry, 1993
- Matrix‐assisted laser desorption time‐of‐flight mass spectrometry of oligonucleotides using 3‐hydroxypicolinic acid as an ultraviolet‐sensitive matrixRapid Communications in Mass Spectrometry, 1993
- Matrix‐assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infraredRapid Communications in Mass Spectrometry, 1992
- Mass spectrometry of DNA mixtures by laser ablation from frozen aqueous solutionRapid Communications in Mass Spectrometry, 1992
- Matrix‐assisted laser desorption mass spectrometry of oligodeoxythmidylic acidsRapid Communications in Mass Spectrometry, 1992
- New developments in biochemical mass spectrometry: electrospray ionizationAnalytical Chemistry, 1990
- Molecular weight determination of underivatized oligodeoxyribonucleotides by positive‐ion matrix‐assisted ultraviolet laser‐desorption mass spectrometryRapid Communications in Mass Spectrometry, 1990
- Ultrafast sequencing of oligodeoxyribonucleo tides by FAB-mass spectrometryNucleic Acids Research, 1982
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977