Vitamin Stability in Diets Sterilized for Germfree Animals

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Abstract
Three diets used routinely in the Lobund Laboratory, containing graded additions of water were steam-sterilized and then analized for thiamine, vitamin B5, calcium pantothenate and riboflavin. In a separate, similar experiment diet L-462 was sterilized and analyzed for vitamin A and carotene. Vitamins, rated in the order of increasing stability in autoclaved air-dried diets, were as follows: thiamine, calcium pantothenate, pyridoxine, vitamin A, carotene and riboflavin. Graded additions of water to the air-dried diets progressively improved the apparent stability of thiamine, pyridoxine and calcium pantothenate, had little effect on vitamin A and riboflavin stability, and decreased the stability of carotene.