Biological Activity of Blood Insulin Complexes Examined by Rat Diaphragm Tissue Assay.

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Abstract
Insulin complexes prepared from human blood showed no significant insulin activity when such preparations were examined by the rat diaphragm tissue assay. After dissociation of insulin from its complex(es) at pH 9.8 insulin-like activity could be demonstrated by this assay. Identical preparations examined by the rat adipose tissue assay showed that tnis tissue could manifest insulin-like activity in the presence of the insulin complex it-self. A difference is thus evident in the mechanism of utilization of the insulin complex(es) by these 2 different tissues.