Use of cultured renal epithelial cells for the study of cisplatin toxicity.
- 1 January 1989
- journal article
- research article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 50 (2) , 247-249
- https://doi.org/10.1254/jjp.50.247
Abstract
The nephrotoxicity of cisplatin was evaluated in an in vitro system with an established cell line of normal rat kidney, NRK-52E. Leakage of enzymes from the cells into the culture medium increased when they were exposed to 1 .mu.M of cisplatin for 72 hr. The level of lipid peroxides increased in the cells after 48 hr of exposure to cisplatin; the increase was more rapid than the enzyme leakage. This culture system can be used to evaluate drug-induced nephrotoxicity and its mechanism.This publication has 11 references indexed in Scilit:
- The use of renal cortical slices from the Fischer 344 rat as an in vitro model to evaluate nephrotoxicity*1Fundamental and Applied Toxicology, 1988
- Effect of N-N'-diphenyl-p-phenylenediamine pretreatment on urinary enzyme excretion in cisplatin nephrotoxicity in rats.The Japanese Journal of Pharmacology, 1988
- Effect of Cisplatin on In Vitro Production of Lipid Peroxides in Rat Kidney CortexThe Japanese Journal of Pharmacology, 1987
- An in vitro screening system for the nephrotoxicity of various platinum coordination complexesCancer Chemotherapy and Pharmacology, 1986
- Urinary Glutathione-S-Transferase in Cisplatin Nephrotoxicity in the Ratcclm, 1986
- Effects of the diuretics mannitol or acetazolamide on nephrotoxicity and physiological disposition of cisplatin in ratsCancer Chemotherapy and Pharmacology, 1984
- CIS-DIAMMINEDICHLOROPLATINUM(II) NEPHROTOXICITY AND ITS RELATIONSHIP TO RENAL GAMMA-GLUTAMYL-TRANSFERASE TRANSPEPTIDASE AND GLUTATHIONE1983
- Alterations in the toxicity of cis-Dichlorodiammineplatinum-II and in tissue localization of platinum as a function of NaCl concentration in the vehicle of administrationToxicology and Applied Pharmacology, 1981
- Assay for lipid peroxides in animal tissues by thiobarbituric acid reactionAnalytical Biochemistry, 1979
- Rapid colorimetric assay of β-galactosidase and N-acetyl-β-glucosaminidase in human urineClinica Chimica Acta; International Journal of Clinical Chemistry, 1976