A rapid, single leaf assay for detecting the presence of ‘S’-male-sterile cytoplasm in maize
- 1 May 1980
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 57 (3) , 97-100
- https://doi.org/10.1007/bf00253876
Abstract
A simple, rapid, and reproducible assay is described for determining unambiguously the presence of S-type cytoplasm in male-sterile and male-fertile (restored) maize lines. Because the assay requires only 0.5 g leaf segment per sample, it is a single plant assay and the plant is not destroyed. Plants at any developmental stage can be used. The assay involves a 30 sec homogenization, 20 min centrifugation, one hour lysis, overnight agarose electrophoresis, 30 min gel staining, and photography of the gel to produce a result in much less than 24 hr. Many samples can be assayed simultaneously. The various assay methods available for classifying maize cytoplasms are compared and discussed.This publication has 18 references indexed in Scilit:
- Low molecular weight circular and linear DNA in mitochondria from normal and male-sterile Zea mays cytoplasmNature, 1980
- Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maizeProceedings of the National Academy of Sciences, 1980
- Variation in mitochondrial translation products associated with male-sterile cytoplasms in maizeProceedings of the National Academy of Sciences, 1978
- Unique DNA associated with mitochondria in the “S”-type cytoplasm of male-sterile maizeProceedings of the National Academy of Sciences, 1977
- Endonuclease recognition sites mapped on Zea mays chloroplast DNAProceedings of the National Academy of Sciences, 1976
- Restriction Endonuclease Analysis of Mitochondrial DNA from Normal and Texas Cytoplasmic Male-Sterile MaizeScience, 1976
- Inhibition of electron transport in maize mitochondria by Helminthosporium maydis race T pathotoxinPhysiological Plant Pathology, 1975
- Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonucleaseJournal of Molecular Biology, 1975
- Diversity and Suitability for Hybrid Production of Different Sources of Cytoplasmic Male Sterility in Maize1Agronomy Journal, 1974
- Cytoplasmic Pollen Sterility in CornAdvances in Genetics, 1965