Comparison between LightCycler Real-Time Polymerase Chain Reaction (PCR) Assay with Serum and PCR-Enzyme-Linked Immunosorbent Assay with Whole Blood Samples for the Diagnosis of Human Brucellosis

Abstract
Background. To overcome some of the limitations of conventional microbiological techniques, polymerase chain reaction (PCR)—based assays have been proposed as a useful tool for the diagnosis of human brucellosis.