Abstract
Antiserum prepared by injecting hemocyanin of Limulus into rabbits reacted with diluted serum of black widow spider. No other antiserum reacted definitely with the spider serum. Others tested were those of Homarus, Cancer, Vivipara, and Busycon. The diluted spider serum contained 0.522 mg. of N and .0054 mg. of Cu per cc. The ratio, 96.7 of N to Cu is not very different from the same ratio for Limulus, 103. The serum protein of the black widow spider is chiefly a hemocyanin, the blood containing probably 3-5% of it.

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