Strains of Bean Yellow Mosaic Virus Compared to Clover Yellow Vein Virus in Relation to Gladiolus Production in Florida

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Abstract
In a survey, 92% of all gladioli tested from the USA and Holland were infected with bean yellow mosaic virus (BYMV). Isolates of BYMV from gladiolus were compared with BYMV isolates from Trifolium pratense (BYMV-204-1), Pisum sativum (BYMV-P), Alpinia zerumbet (BYMV-A), and Freesia refracta (BYMV-F), and a clover yellow vein virus (CYVV) isolated from T. repens (CYVV-P). The gladiolus isolates of BYMV were indistinguishable in host range tests, but differed from BYMV isolates from other hosts and CYVV. In immunodiffusion tests with antisera to BYMV-204-1, CYVV-P, and a gladiolus isolate (BYMV-G) all of the gladiolus isolates reacted identically but could be distinguished by spur formation from each of the other isolates. The G and 204-1 isolates of BYMV were more closely rleated to each other than to CYVV-P in immunodiffusion tests; however, in enzyme-linked immunosorbent assay tests, BYMV-G appeared to be only distantly related to either BYMV-204-1 or CYVV-P. The in vitro translation products of BYMV-G were more similar to those of BYMV-204-1 than to those of CYVV-P. The estimated MW of at least 3 products of CYVV-P differed from the corresponding products of BYMV-G and BYMV-204-1. The gladiolus isolates were closely related to the isolate from T. pratense and distantly related to the isolate from T. repens.