Dynamics of adipogenic promoter DNA methylation during clonal culture of human adipose stem cells to senescence
Open Access
- 29 May 2007
- journal article
- Published by Springer Nature in BMC Cell Biology
- Vol. 8 (1) , 18
- https://doi.org/10.1186/1471-2121-8-18
Abstract
Potential therapeutic use of mesenchymal stem cells (MSCs) is likely to require large-scale in vitro expansion of the cells before transplantation. MSCs from adipose tissue can be cultured extensively until senescence. However, little is known on the differentiation potential of adipose stem cells (ASCs) upon extended culture and on associated epigenetic alterations. We examined the adipogenic differentiation potential of clones of human ASCs in early passage culture and upon senescence, and determined whether senescence was associated with changes in adipogenic promoter DNA methylation.Keywords
This publication has 56 references indexed in Scilit:
- Transcriptional control of adipocyte formationPublished by Elsevier ,2006
- Stable stem cell commitment to the adipocyte lineage by inhibition of DNA methylation: Role of the BMP-4 geneProceedings of the National Academy of Sciences, 2006
- Stable CpG Hypomethylation of Adipogenic Promoters in Freshly Isolated, Cultured, and Differentiated Mesenchymal Stem Cells from Adipose TissueMolecular Biology of the Cell, 2006
- A genetic variant at the fatty acid-binding protein aP2 locus reduces the risk for hypertriglyceridemia, type 2 diabetes, and cardiovascular diseaseProceedings of the National Academy of Sciences, 2006
- Adipogenesis: cellular and molecular aspectsPublished by Elsevier ,2005
- Adipose-derived adult stromal cells heal critical-size mouse calvarial defectsNature Biotechnology, 2004
- Historical claims and current interpretations of replicative agingNature Biotechnology, 2002
- DNA methylation patterns and epigenetic memoryGenes & Development, 2002
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering, 2001