Detection of RNA‐dependent DNA polymerase activity in the Xiphophorus melanoma system
- 3 March 1990
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 188 (2) , 301-308
- https://doi.org/10.1111/j.1432-1033.1990.tb15403.x
Abstract
DNA polymerases have been isolated from muscle and melanoma tissues of Xiphophorus, which are similar to retroviral RNA-dependent DNA polymerases as they prefer RNA to DNA templates. They appear to associate with submicroscopic structures which exhibit a density of about 1.13 g/ml after sucrose-density-gradient centrifugation. The RNA-dependent-DNA-polymerase-like enzymes could be separated from the DNA-dependent DNA polymerases by DEAE-cellulose chromatography. Further purification on phosphocellulose revealed that the muscle enzyme eluted at the void volume and at about 0.6 M KCl, whereas most of the melanoma enzyme eluted at 0.1 M KCl. Comparison of the template primer specificities of the muscle and melanoma enzymes with those of known DNA polymerases showed obvious similarities to the RNA-dependent DNA polymerase isolated from Rous sarcoma virus.Keywords
This publication has 54 references indexed in Scilit:
- Reverse transcription in bacteriaCell, 1989
- Expression of cauliflower mosaic virus reverse transcriptase in yeastNature, 1986
- Demonstration of free reverse transcriptase in the nuclei of embryonic tissues of the Japanese quailBiochemical and Biophysical Research Communications, 1983
- Involvement of reverse transcription in the replication of cauliflower mosaic virus: A detailed model and test of some aspectsCell, 1983
- Purification of protein kinase of mouse sarcoma virus and its effect on reverse transcriptase activityBiochemical and Biophysical Research Communications, 1979
- Occurrence of particle-bound reverse transcriptase in human amniotic fluidBiochemical and Biophysical Research Communications, 1977
- HeLa cell DNA polymerase .gamma.: further purification and properties of the enzymeBiochemistry, 1976
- Poly(2′-O-methylcytidylate)·oligodeoxyguanylate, a template-primer specific for reverse transcriptase, is not utilized by hela cell γ DNA polymerasesBiochemical and Biophysical Research Communications, 1975
- Reverse Transcriptase in Normal Rhesus Monkey PlacentaScience, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970