Distribution of DNA between sister cells during serial subcultivation of human fibroblasts
- 1 March 1982
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 75 (1) , 11-24
- https://doi.org/10.1007/bf00492529
Abstract
The segregation of DNA at the time of cell division was analysed by measuring the DNA contents of cells in mitosis and the incorporation of tritiated thymidine (3H-TdR) on each half of anaphases and telophases. Results suggest that the spreads in the 2C and 4C DNA contents are due to quantitative differences which could originate during semi-conservative DNA synthesis, chromosome assembly and chromosome segregation. This continuous rearrangement of the genome could lead either to a degenerative process or to a differentiation program.This publication has 32 references indexed in Scilit:
- The purification of plasma membranes from WI-38 fibroblasts Effects of ageing on their compositionBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- Aging in vitro and D-glucose uptake kinetics of diploid human fibroblastsJournal of Cellular Physiology, 1981
- Loss of reiterated DNA sequences during serial passage of human diploid fibroblastsCell, 1980
- Repair of X-ray-induced DNA damage in aging human diploid cellsExperimental Cell Research, 1980
- Effect of low dose rate irradiation on the division potential of cells in vitro. VI. Changes in DNA and in radiosensitivity during aging of human fibroblastsMechanisms of Ageing and Development, 1979
- Control of transcription in aging WI38 cells stimulated by serum to divideExperimental Cell Research, 1978
- Age-related decline in the synthesis of glycosaminoglycans by cultured human fibroblasts (WI-38)Mechanisms of Ageing and Development, 1978
- Evidence that transcription changes in ageing cultures are terminal events occuring after the expression of a reduced replicative potentialMechanisms of Ageing and Development, 1978
- DNA-binding proteins in young and senescent normal human fibroblastsExperimental Cell Research, 1975
- Histone acetylation during aging of human cells in cultureBiochemical and Biophysical Research Communications, 1972