EFFECT OF BOVINE GROWTH HORMONE AND A PARTIALLY PURE PREPARATION OF SOMATOMEDIN ON VARIOUS GROWTH PARAMETERS IN HYPOPITUITARY DWARF MICE

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Abstract
The growth-promoting effects of a partially purified preparation of somatomedin [SM] 12.7 U/mg were compared with those of various doses of bovine GH [growth hormone] (5, 20 and 80 .mu.g/day) when injected into hypopituitary dwarf mice. Growth parameters studied were body wt and tail length velocities (calculated as the slope of a regression line fitted to daily measurements against time), uptake of 35SO42- into costal cartilage in vivo and organ weights (heart, liver and kidney). In the 1st experiment SM (6.4 U/day), bovine GH and 0.9% NaCl were injected once daily in a volume of 0.1 ml for 10 days. Treatment with bovine GH promoted a significant dose-dependent increase in body wt and tail length velocities and 35SO42- uptake into costal cartilage in vivo. SM also promoted a significant increase in body wt velocity and 35SO42- uptake, both responses were between that observed with the lowest dose of bovine GH and control values. SM did not promote increase in tail length velocity. Organ weights did not differ significantly between any of the treatment groups when expressed as mg/g body wt. In the 2nd experiment SM (a daily total of 21.6 U/day) and 0.9% NaCl were injected 3 times per day in a volume of 0.033 ml, bovine GH was again injected once daily in a volume of 0.1 ml, and the treatment period was 12 days. As in the 1st experiment all doses of bovine GH and SM promoted a significant increase in body weight velocity. These results are consistent with the SM hypothesis.

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