Conjugal Transfer in Lactic Streptococci of Plasmid-Encoded Insensitivity to Prolate- and Small Isometric-Headed Bacteriophages
- 1 March 1988
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 54 (3) , 777-783
- https://doi.org/10.1128/aem.54.3.777-783.1988
Abstract
Eight of 40 strains of Streptococcus lactis and S. lactis subsp. diacetylactis were able to conjugally transfer a degree of phage insensitivity to Streptococcus lactis LM0230. Transconjugants from one donor strain, S. lactis subsp. diacetylactis 4942, contained a 106-kilobase (kb) cointegrate plasmid, pAJ1106. The plasmid was conjugative (Tra + ) and conferred phage insensitivity (Hsp) and lactose-fermenting ability (Lac) in S. lactis and Streptococcus cremoris transconjugants. The phage resistance mechanism was effective against prolate- and small isometric-headed phages at 30°C. In S. lactis transconjugants, the phage resistance mechanism was considerably weakened at elevated temperatures. A series of deletion plasmids was isolated from transconjugants in S. cremoris 4854. Deletion plasmids were pAJ2074 (74 kb), Lac + , Hsp + , Tra + ; pAJ3060 (60 kb), Lac + , Hsp + ; and pAJ4013 (13 kb), Lac + . These plasmids should facilitate mapping Hsp and tra genes, with the aim of constructing phage-insensitive strains useful to the dairy industry.This publication has 16 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Bacteriophage Resistance Conferred on Lactic Streptococci by the Conjugative Plasmid pTR2030: Effects on Small Isometric-, Large Isometric-, and Prolate-Headed PhagesApplied and Environmental Microbiology, 1986
- Electron microscopic heteroduplex study and restriction endonuclease cleavage analysis of the DNA genomes of three lactic streptococcal bacteriophagesApplied and Environmental Microbiology, 1986
- Conjugal Transfer from Streptococcus lactis ME2 of Plasmids Encoding Phage Resistance, Nisin Resistance and Lactose-fermenting Ability: Evidence for a High-frequency Conjugative Plasmid Responsible for Abortive Infection of Virulent BacteriophageMicrobiology, 1985
- Two plasmid-determined restriction and modification systems in Streptococcus lactisPlasmid, 1984
- Conjugative 40-megadalton plasmid in Streptococcus lactis subsp. diacetylactis DRC3 is associated with resistance to nisin and bacteriophageApplied and Environmental Microbiology, 1984
- Evidence for Plasmid Linkage of Restriction and Modification in Streptococcus cremoris KHApplied and Environmental Microbiology, 1981
- A multiple plasmid-containing Escherichia coli strain: Convenient source of size reference plasmid moleculesPlasmid, 1978
- Improved medium for lactic streptococci and their bacteriophages.1975
- Loss of lactose metabolism in lactic streptococci.1972