Expression of foreign DNA in Chlamydomonas reinhardtii

1 November 1989

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 65 (1-2) , 77-81
https://doi.org/10.1016/0378-1097(89)90369-8

Abstract

A chimeric octopine synthase-neomycin phosphotransferase (ocs-nptII) gene was used to transform Chlamydomonas reinhardtii to kanamycin resistance. Southern hybridization using DNA isolated from one transformant, T6.1, indicated that the entire ocs-nptII gene and at least part of the plasmid were integrated into nuclear DNA. Neomycin phosphotransferase II activity has been detected in T6.1 cell extracts. Northern hybridizations, employing a radiolabeled ocs-nptII sequence, revealed a T6.1 transcript approximately the same size as a homologous transcript isolated from E. coli carrying the nptII gene. Although T6.1 is an extremely rare examiple of a stable C. reinhardtii transformant, its occurence nevertheless indicates that bacterial genes can be expressed in the nucleus of the alga.

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