Electron immunoperoxidase staining in sections of unfixed pure cultures ofStreptococcus mutans
- 31 December 1981
- journal article
- research article
- Published by Medical Journals Sweden AB in Acta Odontologica Scandinavica
- Vol. 40 (5) , 283-287
- https://doi.org/10.3109/00016358209024071
Abstract
Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolme-thacrylate. Uitrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus γ-globulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.Keywords
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