The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation
- 1 September 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 31 (1) , 444-447
- https://doi.org/10.1111/j.2042-7158.1979.tb13550.x
Abstract
A comparison was made of various indices of the hepatic drug-metabolizing apparatus associated with the postmitochondrial supernatant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone-pretreated and 3-methylcholanthrene-pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P-450 and by the activity of NADPH-cytochrome c reductase, 4-dimethylaminoantipyrine N-demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes.This publication has 22 references indexed in Scilit:
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