Determination of methotrexate in untreated body fluids by micellar liquid chromatography

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Abstract
A fast and sensitive method for determination of the antineoplastic drug methotrexate in untreated serum and urine is described. Reversed-phase liquid chromatography with sodium dodecyl sulfate (SDS) micellar mobile phase has been used with direct sample injection. Changes in mobile phase variables such as SDS concentration and/or pH profoundly affected drug retention. Recovery was quantitative and the detection limit (90 nM for a 20 .mu.L sample size) was below the range normally monitored. Multichannel UV detection provided strong evidence regarding peak purity and peak identity.