Polynucleotides. 41. Synthesis and template-directed polymerization of adenylyl(3'-5')adenosine cyclic 2',3'-phosphate
- 8 February 1977
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 16 (3) , 493-498
- https://doi.org/10.1021/bi00622a024
Abstract
Adenylyl(3''-5'')adenosine cyclic 2'',3''-phosphate (A-A > p) was synthesized and its polymerization was attempted under various conditions in the presence of poly(U) and 1,3-propanediamine. Reaction at -20.degree. C for 16 days gave polymerized products (up to the 8-mer) in 15% yield and was proved to be dependent on the template. Reaction at 0.degree. C for 16 days gave more extensive (up to the 10-mer) and more efficient (35%) polymerization. The newly formed phosphodiester linkage was exclusively 2''-5''. These results are discussed in comparison with the monomer-condensation reaction.This publication has 3 references indexed in Scilit:
- Polynucleotides X. Oligonucleotides and their association with polynucleotidesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- Studies on Some Interactions and Reactions of Oligonucleotides in Aqueous Solution*Biochemistry, 1966
- Adenylate oligomers in single- and double-strand conformationJournal of Molecular Biology, 1966