Identity and substrate specificity of human erythrocyte membrane‐bound and cytosolic casein kinases
- 4 November 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 292 (1-2) , 141-144
- https://doi.org/10.1016/0014-5793(91)80852-t
Abstract
The relationship and substrate specificity of the human erythrocyte membrane kinase and casein kinase A were investigated. Based onStaphylococcus aureus V8 protease digestion patterns, the 2 kinases appeared to be structurally homologous. These enzymes also exhibited the same substrate specificity and phosphorylated the same synthetic peptides and domains of ankyrin. Both kinases did not utilize GTP effectively as a substrate and were not inhibited by low concentrations of heparin, suggesting that they were type 1 casein kinases. An analysis of synthetic peptide phosphorylation failed to reveal a specific pattern of recognition of the amino acid sequence surrounding the phosphorylation site.This publication has 25 references indexed in Scilit:
- Protein kinase recognition sequence motifsTrends in Biochemical Sciences, 1990
- Synthetic phosphopeptides are substrates for casein kinase IIFEBS Letters, 1990
- A synthetic peptide substrate specific for casein kinase‐1FEBS Letters, 1989
- Casein Kinase II as a Potentially Important Enzyme Concerned with Signal TransductionCold Spring Harbor Symposia on Quantitative Biology, 1988
- Phosphorylation reduces the affinity of protein 4.1 for spectrinBiochemistry, 1986
- Multisite phosphorylation of glycogen synthase from rabbit skeletal muscleEuropean Journal of Biochemistry, 1985
- Possible identity of a membrane-bound with a soluble cyclic AMP-independent erythorocyte protein kinase that phosphorylates spectrinBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- Different sensitivity of human red cell casein kinases towards glycoaminoglycansBiochemical and Biophysical Research Communications, 1981
- Purification and characterization of a casein kinase from human erythrocyte cytosolBiochemical and Biophysical Research Communications, 1979
- Selective phosphorylation of erythrocyte membrane proteins by the solubilized membrane protein kinasesBiochemistry, 1977