THE ACTION OF DN-ASE ON CELL NUCLEI

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Abstract
Digestion of isolated nuclei from a variety of invertebrate and vertebrate animals by desoxyribonuclease (DNase) is quantitatively described. The results show that (a) not all the DNA can be removed by the enzyme, only a percentage characteristic of the kind of nucleus; (b) no protein is released with the DNA; (c) treatment of the residue with 0.2 [image]HC1 removes the basic protein, rendering the resistant fraction susceptible to digestion with DNase. The conclusion is reached that the fraction of DNA resistant to removal by DNase is a spatially separate component of the total DNA. The resistance to removal may be due to the spatial orientation of the DNA within the nucleus or to the unique mode of combination with protein of the nucleus.

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