Radioimmunoassay Evaluation and Quality Control by Use of a Simple Computer Program for a Low Cost Desk Top Calculator

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Abstract
A simple computer program for the data processing and quality control of radioimmunoassay was presented. It is written for low cost programmable desktop calculator (Helwett Packard 97), which can be afforded by smaller laboratories. The untreated counts from the scintillation spectrometer are entered manually; the printout gives the following results: initial data, logit-log transformed calibration points, parameters of goodness of fit and of the position of the standard curve, control and unknown samples dose estimates (mean value from single dose interpolations and scatter of replicates) together with the automatic calculation of within assay variance and, by use of magnetic cards holding the control parameters of all previous assays, between assay variance.