Effects of 5-bromodeoxyuridine on the ACTH-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.

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Abstract
Cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyurine (BrdU) during their proliferative phase and during ACTH stimulation when nuclear DNA synthesis has almost ceased. Pretreatment with 0.5 .mu.g/ml per day of BrdU inhibited the ACTH-induced differentiation of cortical cells and the secretion of corticosterone and 18-OH-deoxycorticosterone (18-OH-DOC). When nuclear DNA synthesis was suppressed and mitochondrial DNA synthesis was stimulated by ACTH, BrdU addition (30 .mu.g/ml per day) permitted normal ultrastructural differentiation of cortical cells, except that the development of mitochondrial inner membranes was inhibited. Simultaneously mitochondrial 11.beta.- and 18-hydroxylations were strongly inhibited while cytoplasmic 21-hydroxylation was not affected.