Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells
- 24 April 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 247 (2) , 303-306
- https://doi.org/10.1016/0014-5793(89)81357-2
Abstract
The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [3H]thymidine incorporation of 50–60% occurred at 3–300, μM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at > 1 μM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.Keywords
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