Identification of Doxapram Metabolites using High Pressure Ion Exchange Chromatography and Mass Spectroscopy

Abstract
1. High pressure ion exchange chromatography has been used for separation of metabolites of doxapram from whole dog urine. Thin layer chromatography was used for additional separation and clean-up. 2. The structures of the metabolites thus isolated were determined by mass spectroscopy. 3. Seven metabolites were separated and identified.