Direct photoaffinity labeling of soluble GTP‐binding proteins in Dictyostelium discoideum
- 5 May 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 200 (1) , 156-160
- https://doi.org/10.1016/0014-5793(86)80529-4
Abstract
Major cytoplasmic GTP‐binding proteins in Dictyostelium discoideum were identified by direct photoaffinity labeling with [α‐32P]GTP. Three actin‐binding proteins and a protein with an apparent molecular mass of 24 kDa (p24) could be labeled with [α‐32P]GTP. p24 binds to DEAE‐cellulose, behaves like a monomer during gel filtration and was purified to homogeneity by GTP‐affinity chromatography. In comparison to other nucleotide triphosphates the binding of GTP to p24 is highly specific.Keywords
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