Measuring kLa with randomly pulsed dynamic method
Open Access
- 15 April 1991
- journal article
- review article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 37 (9) , 889-893
- https://doi.org/10.1002/bit.260370914
Abstract
This reports on the determination of the overall oxygen transfer coefficient in a mechanically agitated vessel using a randomly pulsed dynamic method. This method consists in exciting the system by randomly switching the inlet gas stream with air or nitrogen with an identical volumetric flow rate. A pseudo‐random binary sequence was used. This procedure is routinely used in process control for the identification of system's transfer function. The pulsed dynamic method gives good reliability (as compared with the traditional gassing‐out method) and reproducibility in water. However, further improvement is needed before it can be used to monitor on‐line thekLaduring a fermentation.Keywords
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